Agilent HPLC Seal Replacement Guide: Compatible, Cost-Effective Alternatives

High-performance liquid chromatography (HPLC) systems, like those from Agilent, rely on precision seals to maintain fluid integrity and analytical accuracy. Over time, these seals degrade, increasing the risk of leaks, pressure instability, or sample contamination. Replacing worn components with compatible, cost-effective alternatives can save your lab money—without sacrificing performance.      

In this guide, we explore the most common Agilent HPLC seal models, provide high-quality replacement options, and show you how to select and maintain them. 

What Are Agilent HPLC Seals and Why Replace Them?  

Seals in Agilent HPLC systems—including piston seals, wash seals, and valve isolation seals—serve critical functions:  

• Piston Seals: Maintain pressure and prevent leakage in the pump head  
• Wash Seals: Keep contaminants away from the main sealing surfaces  
• Isolation Seals: Separate solvent paths inside multi-position valves  

Replacing these seals is part of routine maintenance. Using aftermarket options can reduce costs and improve operational flexibility, especially for labs managing multiple instruments. 

Compatible Seal Models for Agilent Systems  

We offer aftermarket seals for a wide range of Agilent HPLC systems, with equivalent performance to OEM parts. Explore the following direct replacements:  

▶ Piston Seal for Agilent G1313A, G1329A/B – Replaces 0905-1294  

900µL high-pressure seal for 1100 and 1200 series autosamplers. Made with PTFE and 316 stainless steel.  

▶ Piston Seal for Agilent 1090 – Replaces 5062-2494  

Precision-molded piston seal built to OEM standards. Designed for Agilent 1090 series pumps.  

▶ Isolation Seal for Agilent Valve – Replaces 0100-1852  

Carbon-filled PTFE seal for 0101-0921 valve in Agilent 1100 & 1200 systems. Features stainless steel spring.  

▶ Wash Seal Compatible with Agilent – Replaces 0905-1175  

Engineered for outer pump areas to prevent salt crystallization and wear. Compatible with 1050, 1100, 1200, 1220, 1260.  

▶ Piston Seal for Agilent 1100/1200/1260 – Replaces 0905-1420  

PTFE seal suitable for multiple G-series models: G1310, G1311, G1312, G1376, G2226, and G4302.  

▶ Piston Seal Replaces 5063-6589 – For Agilent 1050/1100/1200  

Durable piston seal alternative for legacy and current-generation Agilent systems. 

Why Our Compatible Seals?  

Switching to high-quality aftermarket seals allows your lab to:  

• Maintain system performance with OEM-matching tolerances  
• Save 30–60% on consumables  
• Reduce lead times with faster fulfillment  
• Purchase in small MOQs or bulk  

All seals are made from chemically resistant materials like PTFE and 316 stainless steel or carbon PTFE blends. 

Cost Comparison: OEM vs Aftermarket Seals  

Using compatible parts allows budget reallocation toward research or expansion. Many labs worldwide have switched with zero compromise in performance.  

► Contact Us for a Quote 

Installation Tips & Maintenance Best Practices  

Replacing seals correctly ensures leak-free, long-lasting operation. Here are tips for Agilent systems:  

• Always flush system before disassembly  
• Use clean gloves to avoid oil contamination  
• Gently seat new seals using OEM tools or soft tips  
• After installation, run system at 50% pressure to test seal integrity  

We recommend replacing piston seals every 6–12 months, depending on usage and solvent types.  

HPLC Maintenance Video: Replacing Pump Seals on Agilent 1100/1200/1260

Learn how to properly replace piston seals on your Agilent HPLC system with this official walkthrough. Ideal for lab technicians maintaining G1311A/B, G1312A, and G1361 series pumps.

Video Source: Agilent Technologies –(https://www.youtube.com/watch?v=vFUVHssMnx4&t=35s)

Frequently Asked Questions (FAQ)  

Q: Can I mix OEM and compatible seals in the same pump?  

A: Yes, but for optimal performance and wear balance, we recommend consistent types within a module.  

Q: How do I know which seal I need?  

A: Check your pump model or original OEM P/N. If unsure, send us a photo or spec sheet—we’ll match it for you.  

Q: How long do aftermarket Agilent seals last vs. OEM?

A: Our seals last 6–12 months under normal usage—comparable to OEM performance.

See Also  

Waters HPLC Seal Replacements  

Ready to optimize your Agilent HPLC performance while reducing maintenance costs? Explore our full line of compatible sealing solutions or get in touch with our team at xhh@xhh-smartech.com.